Transgenic Embryo Production
Transgenic Embryo Production - Related Videos
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
04:29
Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos
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2025
This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.
09:48
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
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Cited by 8 •
2010
This video protocol demonstrates a method for generating transgenic Xenopus laevis by introduction of transgenes into sperm nuclei followed by nuclear transplantation into unfertilized eggs.
12:03
The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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Cited by 4 •
2011
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
09:10
Generation of Transgenic Hydra by Embryo Microinjection
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Cited by 34 •
2014
Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
FAQs
The choice among pronuclear microinjection, electroporation, and viral delivery reflects different ways of introducing a genetic sequence into a fertilized egg or early embryo. The overview identifies these as alternative delivery methods, but does not establish that one is universally superior. Their shared purpose is to make the introduced sequence available during subsequent embryonic cell division.
Timing matters because the introduced DNA is present while the embryo undergoes early divisions. As development proceeds, modified cells divide with the embryo, creating the opportunity to examine whether the sequence becomes stably integrated and expressed in resulting offspring. Thus, early-stage manipulation links the initial intervention to later genetic and biological assessment.
Stable integration and transgene expression answer different experimental questions. Screening for integration asks whether the introduced sequence was retained in the offspring's genetic material, whereas screening for expression asks whether that sequence is active. Assessing both prevents researchers from treating DNA presence alone as evidence that the intended biological effect is occurring.
A typical workflow begins by introducing DNA into a fertilized egg or early embryo using an available delivery method. The manipulated embryo is allowed to continue development, then may be transferred to a surrogate for gestation. After offspring are produced, researchers screen them for stable transgene integration and expression.
Embryo transfer connects laboratory manipulation with development in an animal. When a manipulated embryo is transferred to a surrogate, gestation can proceed after the experimental handling stage. The resulting offspring are then evaluated rather than assumed to carry a useful modification, because screening is needed to identify stable integration and expression.
Within biology, this approach supports several distinct lines of investigation. Researchers can examine gene function during development, study how an introduced sequence behaves across inheritance, and investigate disease mechanisms. The same strategy also provides a basis for exploring selected traits in agricultural settings and potential biomedical applications, although particular traits or diseases are not specified here.