Tumor Dissection
Tumor Dissection - Related Videos
04:09
The Pocket-Creation Procedure of Endoscopic Submucosal Dissection for Large Rectal Laterally Spreading Tumors
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Cited by 1 •
2026
This study details a standardized PCM-ESD protocol for resecting large rectal LSTs, aiming to enhance dissection speed and prevent transmural perforation via submucosal tunneling.
06:44
Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
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Cited by 2 •
2021
Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.
07:46
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
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Cited by 19 •
2021
In the age of immunotherapy and single-cell genomic profiling, cancer biology requires novel in vitro and computational tools for investigating the tumor-immune interface in a proper spatiotemporal context. We describe protocols to exploit tumor-immune microfluidic co-cultures in 2D and 3D settings, compatible with dynamic, multiparametric monitoring of cellular functions.
10:13
A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
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Cited by 29 •
2015
Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
FAQs
Preserving tissue architecture allows researchers to interpret selected cells in their original spatial context rather than as disconnected material. During tumor dissection, the chosen region and its cellular material can remain suitable for histology, molecular profiling, or cell analysis. This matters because spatially distinct areas may contain malignant, stromal, immune, or healthy tissue with different biological signals.
Region-specific sampling makes intratumoral differences easier to detect. A specimen can be separated into areas containing predominantly malignant cells or surrounding stromal, immune, or healthy tissue, reducing ambiguity when their signals are analyzed together. In cancer research, this supports more precise investigation of tumor heterogeneity and helps relate regional biology to disease progression, treatment response, or therapeutic resistance.
Tumor dissection can target the boundary between tumor and surrounding normal tissue or distinct regions within the tumor itself. This distinction is important because a tumor sample may contain multiple biological compartments, including malignant cells, stroma, immune cells, and healthy tissue. Separating these areas enables downstream results to be interpreted as region-specific rather than attributed to an undifferentiated specimen.
A workflow begins by identifying the tissue area of interest, then separating that area from surrounding tissue or other tumor regions using visual, microscopic, or instrument-assisted techniques. Researchers aim to isolate the selected material while preserving its architecture and cellular content. The resulting region-specific samples can then undergo histology, molecular profiling, or cell analysis.
Samples obtained through tumor dissection can support histology, molecular profiling, and cell analysis. Histology helps examine tissue structure, while molecular profiling and cell analysis can characterize biological differences among selected regions or tissue compartments. Using these analyses on separated areas improves the ability to connect observed signals with malignant, stromal, immune, or healthy components.
Researchers can compare distinct tumor regions to investigate whether their biological features differ during disease progression or after treatment. Region-specific analysis may reveal variation associated with treatment response or therapeutic resistance that could be obscured when malignant and surrounding tissues are evaluated together. This makes dissection useful for linking local tumor biology with broader cancer behavior.