Hyperglycemia Zebrafish
Hyperglycemia Zebrafish - Related Videos
13:35
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
0 Views •
Cited by 25 •
2017
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
05:49
Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
0 Views •
Cited by 6 •
2021
This protocol noninvasively induces hyperglycemia in zebrafish for up to 8 weeks. Using this protocol, an in-depth study of the adverse effects of hyperglycemia can be made.
01:29
Hyperglycemia
0 Views •
2026
Hyperglycemia is an abnormally high blood glucose level. It is diagnosed by fasting glucose ≥126 mg/dL, 2-hour oral glucose tolerance test (or OGTT) ≥200 mg/dL, random glucose ≥200 mg/dL with symptoms, or HbA1c ≥6.5%. However, HbA1c results may be unreliable in certain conditions, such as anemia or hemoglobinopathies, and the diagnosis should be confirmed unless classic symptoms are present. Postprandial hyperglycemia is typically considered significant when glucose levels exceed 180 mg/dL two...
04:26
Streptozocin Treatment: A Zebrafish Model of Type 1 Diabetes Mellitus
0 Views •
2023
This video describes the technique of creating a zebrafish model for Type 1 diabetes mellitus with the help of the drug Streptozocin, to study the pathophysiology of diabetic complications and metabolic memory.
10:03
A Zebrafish Model of Diabetes Mellitus and Metabolic Memory
0 Views •
Cited by 69 •
2013
Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.
FAQs
Elevated glucose availability can affect cellular signaling, growth, and tissue formation during development. These processes are closely connected because developmental cells must coordinate signals that regulate proliferation and organization as tissues form. By examining how those processes change under hyperglycemic conditions, researchers can connect altered glucose regulation with developmental phenotypes rather than evaluating glucose levels alone.
Studies commonly assess how altered glucose conditions influence growth and the formation of tissues, organs, and blood vessels. These endpoints help reveal whether hyperglycemia affects development broadly or produces more localized abnormalities. In developmental biology, comparing these features with those observed under the corresponding experimental baseline can identify glucose-associated changes in tissue organization and organ development.
Cellular signaling provides a mechanistic link between excess glucose and visible developmental outcomes. A change in glucose availability may influence how cells coordinate growth and tissue formation, which can subsequently appear as abnormalities in developing structures. Examining signaling alongside morphology therefore helps researchers interpret whether a phenotype reflects disrupted developmental regulation rather than an isolated change in appearance.
A study generally uses zebrafish embryos or larvae and introduces elevated glucose through glucose-rich conditions or by inducing impaired glucose regulation. Researchers then examine development under those altered conditions, focusing on cellular signaling, growth, tissue formation, and selected organ or vascular features. The workflow links the experimental metabolic condition to observable developmental outcomes in living animals.
Zebrafish provide several practical advantages for developmental studies: they are small, develop rapidly, and permit optical observation while alive. These features allow researchers to monitor glucose-related phenotypes during early development without relying only on endpoint measurements. The model is therefore suited to efficient investigation of how altered glucose conditions correspond with changes in growth, tissue formation, organs, and vascular development.
These models support investigation of developmental abnormalities associated with metabolic disease, including changes in organ and vascular development. They also provide a platform for testing potential therapeutic strategies by allowing researchers to observe whether experimental interventions alter glucose-related developmental phenotypes. Their use connects metabolic regulation with tissue formation and helps evaluate outcomes in an intact, developing organism.