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Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
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Neuroscience
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JoVE Journal Neuroscience
Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish

DOI:
10.3791/61935-v

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05:49 min

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May 05, 2021

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, , ,
1Department of Biology,American University, 2Center for Behavioral Neuroscience,American University, 3Department of Chemistry,American University

Chapters

  • 00:05Introduction
  • 00:57Preparing the Solution Tanks
  • 02:07Transferring Fish
  • 03:04Post-transfer Solution Preparation
  • 03:53Results: Changes in Blood Glucose, GFAP Levels, and ERG Response in Zebrafish after Induction of Hyperglycemia
  • 05:05Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol noninvasively induces hyperglycemia in zebrafish for up to 8 weeks. Using this protocol, an in-depth study of the adverse effects of hyperglycemia can be made.

Tags

Keywords: HyperglycemiaZebrafishNon-invasiveGlucoseType 2 DiabetesTherapeuticProtocolWater BathHousing TankSolution TankWater-to-waterMannitolAir StoneTank Cleaning

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