Diagnostic Pcr
Diagnostic Pcr - Related Videos
09:57
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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Cited by 11 •
2016
Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
10:21
Microfluidic Applications for Disposable Diagnostics
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Cited by 1 •
2008
In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new diagnostics.
11:00
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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Cited by 11 •
2016
High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.
07:37
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Cited by 10 •
2016
A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
FAQs
Sequence-specific primers determine which environmental target is amplified. They bind to the selected DNA or RNA sequence, giving the reaction a basis for distinguishing a microorganism, genetic marker, or contaminant from other material in the sample. This targeting is central to Diagnostic PCR’s specificity and makes the resulting signal relevant to the question being investigated.
Thermal cycling separates the strands, allows primers to anneal, and then enables polymerase-driven extension. Repeating these stages increases the amount of the selected target until it can be measured through fluorescence or another signal. The cycle sequence therefore links molecular recognition to a detectable result, rather than relying on visual or culture-based identification.
Diagnostic PCR differs from relying solely on culture-based methods because it can detect sequence targets directly in environmental material. Its sensitivity supports finding targets present at low levels, while specificity helps associate the signal with the selected sequence. Together, these properties make results useful for identifying pathogens, contaminants, or genetic markers in environmental surveillance.
An environmental investigation can apply the method to water or soil samples, depending on the surveillance question. The analysis may be directed at a pathogen, contaminant, or genetic marker, and detection is based on the corresponding target sequence. This allows researchers to examine distinct environmental matrices while keeping the molecular readout tied to a defined target.
When should researchers choose Diagnostic PCR for environmental surveillance? It is especially relevant when they need rapid identification of biological targets and timely information about possible hazards. The resulting evidence can support public health assessments and responses to emerging biological hazards, where timely monitoring is important.
Beyond detecting a single pathogen, the approach can support assessment of microbial communities by targeting relevant genetic sequences. It can also help monitor contamination during an environmental investigation. These uses extend the method from one-time identification toward broader evaluation of biological conditions in water or soil and their significance for environmental research.