Permeation Assay
Permeation Assay - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
05:54
Controlled Odor Mimic Permeation Systems for Olfactory Training and Field Testing
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Cited by 1 •
2021
The Controlled Odor Mimic Permeation System is a simple, field-portable, low-cost method of odor delivery for olfactory testing and training. It is constructed of an odorant retained on an adsorbent material and contained inside of a permeable polymer bag allowing controlled release of the odorant vapor over time.
10:47
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
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Cited by 23 •
2011
An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.
10:04
A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity
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Cited by 13 •
2014
Introduction of small molecules to the developing Drosophila embryo offers great potential for characterizing biological activity of novel compounds, drugs, and toxins as well as for probing fundamental developmental pathways. Methods described herein outline steps that overcome natural barriers to this approach, expanding the utility of the Drosophila embryo model.
FAQs
The amount of tracer, drug, or microbial component detected on the opposite side provides a quantitative readout of passage through the cell layer. Measuring its appearance over time helps distinguish limited transfer from increased permeability. In this way, the assay links a numerical transport result to the condition of the biological barrier rather than relying only on visual assessment.
Researchers can compare permeability measurements from exposed and unexposed cell barriers to determine whether inflammation or pathogen contact alters barrier function. A greater appearance of the test substance on the opposite side indicates more passage under the tested conditions, whereas a smaller change suggests less disruption. These comparisons help characterize how host barriers respond during immunological or infectious challenges.
Testing the same barrier system with different microbial components, inflammatory conditions, or candidate treatments allows researchers to compare their effects on permeability. A treatment can be evaluated by whether it reduces the passage associated with a disruptive exposure. This comparative design supports assessment of compounds intended to preserve host defenses, while also showing which challenges produce stronger barrier impairment.
A basic setup uses cells grown on a porous membrane, with the substance of interest added to one side and its appearance monitored on the other. The test material may be a tracer, drug, or microbial component, depending on the research question. Quantifying transfer over time produces the measurement needed to compare barrier behavior across experimental conditions.
A tracer can provide a general indicator of passage, whereas a drug can be used to examine transport relevant to treatment studies. A microbial component places the measurement in an infection-related context. Because each substance represents a different experimental question, the selected material determines whether the assay emphasizes barrier integrity, compound movement, or responses to pathogen-associated exposure.
They can assess epithelial or endothelial permeability during inflammation or pathogen exposure, compare how strongly different challenges disrupt a barrier, and evaluate candidate treatments that aim to preserve host defenses. The resulting quantitative comparisons help connect immune or infectious conditions with changes in barrier function, making the assay useful for both mechanism-focused studies and treatment evaluation.