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A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity
JoVE Journal
Biology
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JoVE Journal Biology
A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity
DOI:

10:04 min

July 13, 2014

Chapters

  • 00:05Title
  • 02:21Preparing the Flies and Materials
  • 03:41Staging and Processing Embryos
  • 05:39Dying and Developing Permeabilized Embryos
  • 07:46Robustness and Methylmercury Treatment
  • 09:26Conclusion

Summary

Automatic Translation

Introduction of small molecules to the developing Drosophila embryo offers great potential for characterizing biological activity of novel compounds, drugs, and toxins as well as for probing fundamental developmental pathways. Methods described herein outline steps that overcome natural barriers to this approach, expanding the utility of the Drosophila embryo model.

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