Mir-122
Mir-122 - Related Videos
09:58
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Cited by 2 •
2017
This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
10:10
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Cited by 25 •
2017
This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
03:58
Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System
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2025
Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a...
FAQs
Its pairing with complementary sequences, commonly in a messenger RNA’s 3′ untranslated region, can change how long that transcript remains available or how much protein it produces. This creates post-transcriptional control of gene expression, allowing miR-122 to influence hepatic processes through the stability and translation of multiple target transcripts rather than through a single gene.
miR-122 is closely linked to the liver’s normal regulatory environment and to lipid metabolism. Changes in its activity can therefore affect biological programs associated with hepatic function, while abnormal regulation may contribute to disease biology. Studying this relationship helps connect molecular changes in miR-122 with broader liver disorders rather than viewing it as an isolated molecular marker.
Inhibition reduces miR-122 activity, whereas restoration aims to recover or increase activity when it is insufficient. These strategies address opposite regulatory states and may produce different biological consequences in liver disease. Researchers investigate both approaches because the desired intervention depends on whether altered miR-122 activity is contributing to viral replication, inflammation, fibrosis, or tumor-related biology.
miR-122 is studied in relation to viral replication as well as lipid metabolism, inflammation, fibrosis, and hepatocellular carcinoma. Its regulatory effects on messenger RNAs provide a possible molecular connection among these processes. Consequently, changes in miR-122 may be relevant not only to viral disease, but also to the progression and biological behavior of chronic liver disorders.
Researchers examine changes in miR-122 abundance in blood or liver tissue and relate those measurements to liver disease biology. The value of this approach comes from connecting a measurable molecular change with conditions such as viral infection, inflammation, fibrosis, or hepatocellular carcinoma. Such measurements are studied as potential biomarkers, while interpretation depends on the disease context.
Therapeutic interest in chronic hepatitis C arises from miR-122’s relationship to viral replication. Strategies that inhibit or restore its activity are investigated to determine whether changing this regulatory pathway can influence infection-related disease biology. This work also illustrates how a liver-enriched microRNA can serve as a therapeutic target, rather than only as an observational biomarker.
In fibrosis and hepatocellular carcinoma, researchers ask whether altered miR-122 regulation reflects or contributes to disease-related molecular changes. Its links to inflammation, hepatic function, and gene-expression control make it relevant across several stages of liver pathology. Studies may therefore examine miR-122 in tissue or blood to connect abundance patterns with these disease processes.