Slit Lamp Microscopy
Slit Lamp Microscopy - Related Videos
11:20
Fabrication and Visualization of Capillary Bridges in Slit Pore Geometry
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Cited by 4 •
2014
A procedure for creating and imaging capillary bridges in slit-pore geometry is presented. The creation of capillary bridges relies on the formation of pillars to provide a directional physical and chemical heterogeneity to pin the fluid. Capillary bridges are formed and manipulated using microstages and visualized using a CCD camera.
06:57
Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
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Cited by 10 •
2015
Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
05:03
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
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Cited by 11 •
2018
This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.
14:11
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
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Cited by 10 •
2014
This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.
FAQs
The focused beam creates an optical section by restricting illumination to a narrow region of the eye. When the beam is angled relative to the viewing path, different transparent and anterior structures can be examined with greater separation in the observed image. This selective illumination helps distinguish localized changes from findings that might be obscured by broad, undirected lighting.
Narrowing the slit concentrates light into a thin plane, while changing its angle alters which ocular surfaces and internal boundaries are emphasized. Together, these adjustments let the examiner inspect the cornea, anterior chamber, lens, conjunctiva, and related tissues as separate optical sections. The approach is useful when structural changes need precise localization during examination.
Magnification enlarges fine structural detail, allowing subtle changes in the examined tissues to be viewed directly. Combined with focused illumination, it supports high-resolution assessment rather than relying only on the eye’s overall appearance. Because imaging occurs in real time and without an invasive intervention, the method can support immediate clinical observation and repeated disease monitoring.
The examination centers on directing a focused, adjustable beam toward the eye while viewing the illuminated tissues under magnification. By narrowing and angling the beam, the examiner can assess transparent and anterior structures through successive optical sections. This workflow provides real-time visual information about structural changes in the cornea, anterior chamber, lens, conjunctiva, and related tissues.
Its neurological relevance comes from connecting visible ocular findings with nervous system function. Examination may reveal abnormalities associated with neural and visual disorders, including changes that reflect damage along the optic pathway. This makes ocular imaging useful for research and clinical assessment when investigators need to relate eye structure to the condition of visual neural systems.
The method provides high-resolution, real-time observations of changes in anterior and transparent eye tissues. These observations can support clinical examination, follow structural changes over time, and contribute to research linking ocular anatomy with nervous system function. In neuroscience, that information helps investigators study how ocular signs correspond to visual or neural disorders without requiring an invasive imaging approach.