Stereotaxic Syringe Pump
Stereotaxic Syringe Pump - Related Videos
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
00:08
Survivable Stereotaxic Surgery in Rodents
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Cited by 50 •
2008
The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
12:45
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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Cited by 22 •
2012
Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.
04:12
Stereotaxic Transplantation of Neural Cell Suspension into a Mouse with Traumatic Brain Injury
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2025
Source: Furmanski, O., et al. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (2019)In this video, human-induced pluripotent stem cell-derived neural cells are stereotaxically injected into the gray matter-white matter border of the deep cortex in a mouse model of traumatic brain injury. The transplanted cells integrate into the tissue, promoting functional recovery and circuit...
FAQs
The motor advances the syringe plunger in calibrated movements, allowing researchers to regulate both the amount of solution delivered and the speed of infusion. This control is especially important when administering minute volumes at very low flow rates. Consistent plunger movement reduces variation between experiments and helps investigators compare neural, behavioral, or disease-related outcomes across subjects.
Stereotaxic coordinates guide the needle to a defined anatomical location within the brain. The apparatus provides three-dimensional positioning, while the pump controls delivery after the target is reached. Separating spatial placement from fluid control helps researchers administer a solution to the intended region and reduce off-target exposure, which is essential when studying localized neural circuits or disease mechanisms.
Very low flow rates help limit tissue disruption during administration of a solution into the brain. They also support controlled delivery of small volumes, making the experimental manipulation more consistent. This matters when researchers evaluate localized effects, because excessive mechanical disturbance or variable infusion conditions could complicate interpretation of changes in neural activity, behavior, or therapeutic response.
The stereotaxic frame establishes the needle's position using three-dimensional coordinates, whereas the syringe pump governs calibrated plunger movement. Together, these components coordinate where a solution is delivered with how quickly and how much is administered. This division of functions supports reproducible experimental manipulations by controlling both anatomical targeting and infusion conditions rather than relying on either factor alone.
A basic workflow combines anatomical positioning with calibrated fluid delivery. Researchers use the stereotaxic apparatus to place the needle at the selected brain coordinates, then operate the motor-driven syringe to administer the chosen solution at a controlled rate and volume. Maintaining these settings across experiments improves comparability and supports consistent interpretation of localized manipulations.
The system can deliver drugs, viral vectors, tracers, and other solutions into selected brain regions. The appropriate choice depends on the experimental question: drugs can support neural manipulation, viral vectors can enable targeted experimental strategies, and tracers can help investigate neural pathways. Controlled administration links the delivered substance to a defined anatomical site for circuit and disease studies.
Researchers use stereotaxic syringe pumps when an experiment requires a localized and reproducible brain manipulation. Applications include studies of neural circuits, behavior, disease mechanisms, and therapeutic strategies. By combining defined anatomical targeting with controlled infusion, the approach helps relate a delivered substance to subsequent experimental outcomes while reducing uncertainty caused by inconsistent placement or delivery.
Accurate targeting and consistent infusion conditions make it easier to attribute observed effects to the intended brain region and administered solution. Reduced off-target delivery strengthens comparisons among experimental subjects and groups. In neuroscience, this supports clearer evaluation of changes related to neural circuits, behavior, disease mechanisms, or potential therapeutic strategies, although interpretation still depends on the experimental design.