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Chapters
- 00:05Title
- 02:11Isolation of E. coli Co-transformants
- 04:19Co-expression of α and ε Subunits of E. coli DNA Polymerase III
- 06:20Co-expression of α Subunit of Deinococcus radiodurans DNA Polymerase III and ε Subunit of E. coli DNA Polymerase III
- 06:49Gel Filtration Chromatography
- 08:09Mutation Analysis of Populations Co-expressing the Wild-type ε Subunit of E. coli DNA Polymerase III and the Mutagenic εD12A Variant
- 10:43Results: Effective Protein Co-expression in E. coli
- 11:55Conclusion
Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.
