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Chapters
- 00:05Title
- 00:34Formation of Cell Spheroids by Hanging Drop Culture
- 02:01Embedding Spheroids in a Three-dimensional (3D) Matrix
- 03:38Analyzing Spheroid Cell Invasion
- 04:19Results: Sphere Forming and Invasive Abilities in a Cancer Cell Line Panel
- 04:51Conclusion
This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.
