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JoVE Journal Medicine
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
 

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Article doi: 10.3791/53409
November 20th, 2015 •
1, 2, 1, 1
1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, 2Department of Biomedical Sciences, University of Minnesota

Summary
Automatic Translation
November 20th, 2015

This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.

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