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A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
JoVE Journal
Medicine
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JoVE Journal Medicine
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

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05:34 min

November 20, 2015

DOI:

05:34 min
November 20, 2015

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Summary

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This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.

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