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JoVE Journal
Medicine

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A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
 

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Article DOI: 10.3791/53409
November 20th, 2015

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Summary November 20th, 2015

This method evaluates cancer cell invasion from spheroids into a surrounding 3D matrix. Spheroids are generated via the hanging drop culture method and then embedded in a matrix comprised of basement membrane materials and type I collagen. Invasion out of the spheroids is subsequently monitored.

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