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JoVE Journal Neuroscience
Dyrking i Vivo-liker Murine astrocyttene ved hjelp av rask, enkel og rimelig AWESAM-protokollen
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Dyrking i Vivo-liker Murine astrocyttene ved hjelp av rask, enkel og rimelig AWESAM-protokollen

Article DOI: 10.3791/56092-v • 07:56 min • January 10th, 2018
January 10th, 2018 •
1,2,3, 3
1Chemical Biology, Chemistry Research Laboratory, University of Oxford, 2Department of Physiology, Anatomy, and Genetics, University of Oxford, 3Trans-synaptic signaling, European Neuroscience Institute

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AWESAM protokollen beskrevet her er optimal for dyrking murine astrocyttene isolert fra andre hjerneceller på en rask, enkel og rimelig måte. AWESAM astrocyttene utstilling spontan Ca2 + signalering morfologi og gene uttrykket profiler ligner astrocyttene i vivo.

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Nevrobiologi problemet 131 astrocyttene monokultur cortex hippocampus mus rotte kalsium vesicle synaptogenesis medium
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