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JoVE Journal Biochemistry
Site specifieke Lysine acetylatie van histonen voor nucleosoom reconstitutie met behulp van genetische code uitbreiding in Escherichia coli
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Site specifieke Lysine acetylatie van histonen voor nucleosoom reconstitutie met behulp van genetische code uitbreiding in Escherichia coli

Article DOI: 10.3791/62113-v • 07:26 min • December 26th, 2020
December 26th, 2020 •
1, 1
1Department of Chemistry, Texas A&M University

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Hier presenteren we een methode om geacetyleerde histoneiwitten uit te drukken met behulp van genetische code-uitbreiding en gereconstitueerde nucleosomen in vitro te assembleren.

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Biochemie nucleosoom acetylatie pyrrolysine genetische code-uitbreiding post translationele modificaties eiwitmodificaties histonen
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