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Immunology and Infection

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Purificazione ed espansione di cellule T Natural Killer invarianti di topo per studi in vitro e in vivo

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Descriviamo un protocollo rapido e robusto per arricchire le cellule T natural killer invarianti (iNKT) dalla milza di topo ed espanderle in vitro a numeri adatti per studi in vitro e in vivo.

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Immunologia e infezione Numero 168 cellule iNKT espansione topo Vα14 CD1d perline anti-CD3/CD28 attivazione linfociti
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