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Forberedelse af rotteskeletmuskelhomogenater til nitrat- og nitritemålinger
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Forberedelse af rotteskeletmuskelhomogenater til nitrat- og nitritemålinger

Article DOI: 10.3791/62427-v • 07:19 min • July 29th, 2021
July 29th, 2021 •
*1, *1, 2, 2, 2, 1, 1
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus, University of Exeter
* These authors contributed equally

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Vi præsenterer protokoller for tre forskellige metoder til homogenisering af fire forskellige muskelgrupper af rotteskeletmuskelvæv for at måle og sammenligne niveauerne af nitrat og nitrit. Desuden sammenligner vi forskellige prøvevægte for at undersøge, om vævsprøvestørrelse påvirker resultaterne af homogenisering.

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Biologi udgave 173 nitrat nitrit nitrogenoxid skeletmuskulatur vævshomogenisering
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