Gamma H2a.x Chip
Gamma H2a.x Chip - Related Videos
01:32
Evoked Gamma Oscillations for the Treatment of Alzheimer’s Disease
0 Views •
2025
Begin with a patient diagnosed with Alzheimer’s disease, wearing a gamma therapy stimulation device.The device consists of an eye-set and headphones.Using the controller, activate the device to deliver auditory and visual stimulation.The visual component emits flickering light at a gamma frequency, while the auditory component produces synchronized sound pulses.Gamma oscillations are brain waves generated by synchronized neuronal firing, crucial for cognition and memory.In AD, these...
05:47
Intravital Visualization of Gamma Delta Intraepithelial Lymphocytes
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2025
This video demonstrates the visualization of γδ intraepithelial lymphocytes (IELs) in transgenic mice using confocal microscopy. The surgical procedure involves creating a perforation line and making a longitudinal incision to expose the intestinal mucosa and villi. The transgenic mice express reporter proteins exclusively in γδ IELs, allowing their tracking. Intravital imaging captures the dynamic movement of green fluorescence-labeled γδ IELs along the intestinal epithelial cell's basement...
09:46
Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
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2025
We present a rapid and efficient method to detect common fragile site breaks through native γH2A.X chromatin immunoprecipitation (ChIP). This approach significantly reduces both the time and labor associated with traditional γH2A.X ChIP assays while maintaining high reproducibility and reliability of results.
09:04
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons
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Cited by 3 •
2016
The pedunculopontine nucleus (PPN) is located in the brainstem and its neurons are maximally activated during waking and rapid eye movement (REM) sleep brain states. This work describes the experimental approach to record in vitro gamma band subthreshold membrane oscillation in PPN neurons.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
FAQs
The antibody against γ-H2A.X selectively enriches nucleosomes carrying the phosphorylated histone mark from fragmented chromatin. DNA recovered from this enriched fraction represents genomic regions associated with that signal, while the resulting PCR or sequencing data identify their locations. This connection between antibody selectivity and DNA recovery allows biochemical chromatin changes to be examined at specific genomic loci.
Fixation preserves chromatin-associated relationships at the time cells are collected, whereas fragmentation makes the chromatin suitable for immunoprecipitation and locus-level analysis. Together, these steps help retain the distribution of γ-H2A.X while producing DNA fragments that can be recovered after antibody enrichment. The resulting material supports comparison of damage-associated chromatin patterns between experimental conditions.
Differences in recovered γ-H2A.X-associated DNA can indicate that DNA-damage patterns or repair-associated chromatin changes differ between conditions. Comparing signal at genomic regions helps connect a biochemical response with particular loci rather than viewing damage only as a global cellular measurement. This makes the assay useful for examining how genome damage and repair processes vary across experiments.
A typical workflow fixes cells, fragments their chromatin, and uses an antibody against γ-H2A.X to enrich marked nucleosomes and the DNA associated with them. The recovered DNA is then identified by PCR for selected regions or by sequencing for broader genomic analysis. Researchers can compare these measurements across conditions to evaluate changes in damage-associated chromatin.
PCR is suitable when the analysis focuses on selected genomic regions and the goal is to compare γ-H2A.X-associated DNA at defined loci. Sequencing provides a broader view by identifying recovered DNA across the genome. Thus, the choice depends on whether the experiment asks a targeted question about particular regions or seeks a wider map of damage-associated chromatin.
The assay links a biochemical histone modification to its genomic location, providing a way to study genome damage in chromatin context. Applications described for this approach include comparing DNA-damage patterns, assessing repair-associated chromatin changes, and investigating genome stability and DNA repair. It also supports cancer biology studies where altered damage responses can be evaluated across experimental conditions.