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Current Methods for Assessing Reactions to DNA Damage Response
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Guest Editors

Songbai Liu

Songbai Liu

Suzhou Vocational Health College, Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Key Laboratory of Medical Biotechnology

Prof. Dr. Songbai Liu is head of Suzhou Key Laboratory of Medical Biotechnology and one of the research direction Leaders in Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology. His main research interests are mechanistic studies on tumorigenesis and drug resistance, DNA damage events as biomarkers for disease diagnosis and prognosis, and targeted inhibitor screening.He is also the Jiangsu Province “Qinglan Program” Academic Leader, Jiangsu Province “333 Program” High-Level Talent, and Jiangsu Province Youth High-level Medical Talent. He is a member of the Editorial Board of the Journal of Molecular and Clinical Oncology, BIOCELL, Medicine International and a guest reviewer editor of Frontiers in Oncology. He has published more than 60 academic papers and received 21 granted patents.

Min Li

Min Li

Hunan Provincial Maternal and Child Health Care Hospital, University of South China; National Health Commission Key Laboratory of Birth Defect Research and Prevention, Institute of Biochemistry and Molecular Biology, Hengyang Medical school

Prof. Dr. Min Li is a professor at Institute of Biochemistry and Molecular Biology, Hengyang Medical school, University of South China. Her main research aims to understand how defects in DNA synthesis and repair contribute to cancer pathogenesis and aging. Specially, the main project of her laboratory focus on transcription-replication conflict associated with genome instability. Dr. Min Li is also a core member of National Health Commission Key Laboratory of Birth Defect Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha.

Haitao Sun

Haitao Sun

Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Plant Development

Prof. Dr. Haitao Sun is a Principal Investigator of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College. Her main research interests are mechanistic studies on DNA replication and DNA damage response, sister chromatid cohesion, tumorigenesis, tumor drug resistance and anti-cancer activities of medicinal plants. She is also on the list of National of High-Level of Talent Program. She is a member of Chinese Society for Cell Biology. She has published more than 10 academic papers in top journals, such as Science, Acta Pharmaceutica Sinica B, Trends Cell Biology, and received 3 patents. 

Di Xin

Di Xin

Zhejiang University School of Medicine, the First Affiliated Hospital, MOE Joint International Research Laboratory of Pancreatic Diseases, Zhejiang Provincial Key Laboratory of Pancreatic Disease, Department of Hepatobiliary and Pancreatic Surgery

Prof. Dr. Di Xin is a Principal Investigator of Zhejiang Provincial Key Laboratory of Pancreatic Disease and MOE Joint International Research Laboratory of Pancreatic Diseases at the First Affiliated Hospital of Zhejiang University School of Medicine. His main research interests are studies on the molecular mechanism of DNA damage repair and tumor development, and the role and molecular mechanism of molecules that maintain genomic stability in innate immunity. Dr. Di Xin is a core member of Hangzhou Leading Innovation and Entrepreneurship Team, a member of Chinese Association of Pancreatology, and a member of Chinese Society for Cell Biology, and a member of is DDRHD (the international symposium on DNA Damage Response & Human Disease) of the Chinese Society of Biochemistry and Molecular Biology. 

Collection Overview

Cells respond to genotoxic stress through complex protein pathways known as the DNA damage response (DDR). This DDR system ensures genome stability by recognizing DNA damage, recruiting response-related proteins, performing cleavage and gap filling, and causing DNA damage-related cell cycle arrest and cell death. These response events significantly impact various human diseases, notably cancer, neurodegenerative disorders, immune deficiencies, and aging.

The Methods Collection aims to provide a comprehensive resource for the research community, offering detailed methods and protocols related to studying the DNA damage response. Some examples of methods included in this collection are as follows:

  • Evaluating the occurrence and degree of different types of DNA damage, including DNA base damage, DNA nucleotide damage, and DNA strand damage, using the comet assay, fluorescence in situ hybridization (FISH), and immunofluorescence staining
  • Assessing the activity levels and interactions of key DNA repair enzymes (such as PARP, FEN1, and APE1) through in vitro enzyme activity analysis or immunoprecipitation technology. Quantitatively analyzing the expression of genes related to DNA repair using quantitative PCR (qPCR) or fluorescence quantitative PCR technology
  • Evaluating the activity and degree of DNA damage response by detecting the expression levels of DNA damage response markers (such as γH2AX and 53BP1) through immunofluorescence staining or immunoblotting
  • Investigating cell cycle changes and cell death pathways using flow cytometry to detect biological changes in cells responding to DNA damage

Articles

Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells
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Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells

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Cited by 4

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2024

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Abstracts

Autophagy-Related Signature Identifies ALDH1A3 as a Biomarker for Immunity and Progression in Gastric Cancer

Ruipeng Wang1,

Jinjing Xu1,

Hao Zhang1,

Qiang Huo1,

Qiange Zhang2,

Yunwen Tao*3,

Ling Gao*1

1Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China,

2Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou, China,

3Department of Clinical Nutrition, Second Affiliated Hospital of Soochow University, Suzhou, China

Live cell imaging of bacterial DNA for assessing DNA damage potential of antibiotics, and pesticides

Sutharsan Govindarajan*1

1Department of Biological Sciences, School of Engineering and Sciences (SEAS), SRM University-AP

EIF4EBP1 in Breast Cancer: Translational Control, Regulatory Networks, and Therapeutic Frontiers

Weijia Liu1,

Lili Hou2,

Dandan Tang3,

Haiyu Mou4,

Weijie Wang5,

Song-Bai Liu*6,

Xiaohua Li*2

1College of Life Science, North China University of Science and Technology, Bohai Avenue 21,Tangshan 063210, China; Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Kehua Road 28, Suzhou 215009, China,

2Thyroid and breast surgery, Wuzhong people’s Hospital of Suzhou City, Suzhou 215128, China,

3School of Chemistry and Life Science, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou 215009, China,

4Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Kehua Road 28, Suzhou 215009, China,

5College of Life Science, North China University of Science and Technology, Bohai Avenue 21,Tangshan 063210, China,

6College of Life Science, North China University of Science and Technology, Bohai Avenue 21,Tangshan 063210, China; Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Kehua Road 28, Suzhou 215009, China; School of Chemistry and Life Science, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou 215009, China

DNA Damage Repair Systems in 5-Fluorouracil Resistance of Colorectal Cancer: Mechanistic Insights and Therapeutic Intervention Strategies

Dandan Tang1,

Yi Yu2,

Ping Xu3,

Xiaoxiao Wang4,

Song-Bai Liu*4

1School of Chemistry and Life Science, Suzhou University of Science and Technology, Suzhou, China,

2Department of Traditional Chinese Medicine Anorectal Surgery, The Second People's Hospital of Kunshan City, Suzhou, China ,

3Department of Clinical Laboratory, The Fifth People’s Hospital of Suzhou, The Affiliated Infectious Diseases Hospital, Suzhou Medical College of Soochow University, Suzhou, China,

4Jiangsu Province Engineering Research Center of Development and Translation of Key Technologies for Chronic Disease Prevention and Control, Suzhou Vocational Health College, Suzhou, China