In Vivo Liver Engineering
In Vivo Liver Engineering - Related Videos
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
13:27
A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
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Cited by 1 •
2018
We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
09:29
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
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Cited by 31 •
2014
Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
FAQs
Successful In Vivo Liver Engineering requires coordination rather than reliance on a single component. Biomaterials can provide structural support, cells can contribute to tissue formation, gene delivery can modify relevant biological activity, and local microenvironmental control can guide growth. Their combined design must support cell survival and organization while allowing the developing tissue to integrate with the host.
Vascularization and host integration are linked indicators of whether engineered liver tissue can become part of the surrounding biological system. The strategy must encourage new blood vessel development while supporting structural incorporation into the host. If these processes are not coordinated with cell survival and tissue organization, growth may not translate into durable, functional repair.
A successful construct must preserve liver-specific activities, especially metabolism and detoxification, rather than producing tissue growth alone. These functions provide an important measure of biological performance during and after repair. Design decisions involving cells, biomaterials, gene delivery, and the local microenvironment therefore need to support both tissue development and sustained functional activity.
Researchers can treat these elements as complementary parts of one design: biomaterials establish a supportive structure, cells provide a biological contribution, gene delivery influences selected processes, and local environmental modulation guides tissue behavior. The combination is then judged by whether it promotes growth, vascularization, host integration, immune compatibility, and liver-specific function rather than by any component alone.
Evaluation should extend beyond visible tissue growth. Relevant outcomes include cell survival, structural organization, vascularization, integration with the host, immune responses, and preservation of metabolism and detoxification. Long-term functional performance is especially important because an approach may encourage early regeneration yet fail to maintain liver-specific activity or stable tissue behavior over time.
The approach is relevant when liver failure or tissue loss creates a need for repair or regeneration and researchers are investigating alternatives to transplantation. It can also provide a platform for evaluating therapeutic mechanisms in a living system. Its broader value lies in linking engineered tissue growth with host responses and liver-specific performance.
Within bioengineering, this area combines material design, cellular strategies, gene delivery, and control of the local biological environment to address a complex organ-level problem. The scientific challenge is not only producing new tissue, but coordinating immune responses, organization, vascularization, integration, and sustained liver function. These priorities connect engineering decisions with clinically relevant regeneration goals.