Toxicology Screening
Toxicology Screening - Related Videos
11:38
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
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Cited by 23 •
2016
The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
08:59
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
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Cited by 3 •
2020
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Cited by 7 •
2016
This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
FAQs
Concentration-dependent changes help distinguish weak effects from responses that increase as exposure rises. Screening can measure outcomes such as cell viability, barrier integrity, or biochemical biomarkers across defined concentrations. This pattern gives researchers a more informative basis for evaluating potential hazards than observing a single exposure level, and it can help identify conditions requiring closer investigation.
Different endpoints capture different biological consequences. Cell viability indicates whether exposed cells remain functional or survive, while barrier integrity reflects whether a tissue-like boundary has been disrupted. Biochemical biomarkers can provide additional evidence of cellular stress or injury. Selecting several complementary responses allows a screening strategy to examine toxicity from more than one biological perspective.
Engineered models provide controlled systems for examining how a substance affects cells or tissues without immediately relying on whole organisms. In bioengineering, these models may support testing of biomaterials, drug candidates, and medical devices. When an in vitro model has been validated, it can contribute to safety assessment while reducing reliance on animal studies.
High-throughput assays enable many conditions or substances to be evaluated efficiently, making them useful for broad screening and comparison. Organ-on-chip systems offer engineered model environments that can reveal mechanisms of injury in a more tissue-focused setting. Together, these approaches can connect initial hazard detection with more detailed analysis of how a response develops.
A screening workflow begins by selecting the substance and an appropriate test system, such as cells, tissues, or an engineered model. The system is exposed to defined concentrations, after which researchers measure outcomes including viability, barrier integrity, or biochemical biomarkers. Comparing dose-dependent responses helps identify harmful effects and determine which candidates or materials merit further evaluation.
The approach is especially useful during development of biomaterials, drug candidates, and medical devices, when safety information can guide design decisions before clinical or environmental exposure. Results may identify harmful effects, indicate possible injury mechanisms, and support safer material design. Screening therefore connects biological testing with practical development decisions in therapeutic and device research.