Arabidopsis Embryo Isolation
Arabidopsis Embryo Isolation - Related Videos
08:05
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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Cited by 17 •
2013
We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.
10:24
A Method for Characterizing Embryogenesis in Arabidopsis
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Cited by 11 •
2017
This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
05:41
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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Cited by 27 •
2017
This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.
09:49
Isolation and Purification of Kinesin from Drosophila Embryos
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Cited by 5 •
2012
This is a protocol to isolate active full length Kinesin from Drosophila embryos for single-molecule biophysical studies. We show how to collect embryos, make the embryo lysate, and then polymerize microtubules (MTs). Kinesin is purified by immobilizing it on the MTs, spinning down the Kinesin-MT complexes, and then releasing the kinesin from the MTs via ATP addition.
FAQs
Isolation exposes the embryo directly, reducing the masking effects of surrounding seed or ovule tissues. Researchers can therefore examine pattern formation and cell division more closely than they could in an intact structure. This separation is especially useful when developmental changes must be attributed to the embryo itself rather than inferred through maternal tissues.
The culture environment supplies water, mineral nutrients, and sugars that support growth after embryos leave the seed or ovule. Because these components can be provided under controlled conditions, researchers can investigate how available resources affect development. The same system also permits examination of hormonal and environmental influences on embryonic growth.
The technique makes early developmental events easier to observe, including changes in cell division and the establishment of embryonic patterns. Direct access also helps researchers follow developmental responses under defined culture conditions. These observations provide a clearer view of early plant development than analyses based only on embryos enclosed within surrounding tissues.
Isolated embryos provide a direct experimental system for examining gene function during embryogenesis while separating the embryo from much of its surrounding maternal context. Researchers can compare developmental behavior under controlled culture conditions and assess whether observed effects are associated with embryonic processes or maternal influences. This supports mechanistic studies in Arabidopsis biology.
A typical workflow begins by dissecting an ovule or seed with fine tools, releasing the developing embryo from surrounding maternal tissues, and transferring it to sterile culture medium. The medium contains water, mineral nutrients, and sugars to support continued growth. Researchers then examine development under controlled conditions, focusing on embryonic structure and responses.
Embryo isolation is useful when researchers need to remove a developing embryo from its original seed or ovule environment and maintain it separately in culture. The controlled medium can support further growth while allowing direct observation. In this way, the approach contributes to embryo rescue investigations as well as broader studies of early development.
Cultured embryos can reveal how embryonic growth changes in response to hormones and environmental conditions, while also supporting analysis of pattern formation and cell division. They provide material for investigating gene function and maternal effects in a controlled setting. These outcomes connect cellular developmental observations with broader questions in plant biology.