Dextran Coupled Dye
Dextran Coupled Dye - Related Videos
01:11
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
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2023
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
12:00
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Cited by 9 •
2015
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
07:35
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Cited by 8 •
2011
This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.
06:47
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
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Cited by 5 •
2012
We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.
FAQs
Dextran-coupled dyes are commonly internalized by fluid-phase endocytosis, in which surrounding fluid and dissolved conjugate enter the cell together. After uptake, the labeled material remains within intracellular compartments, so its fluorescence reports where the internalized fluid has traveled rather than simply marking the plasma membrane. This supports analysis of endocytic trafficking over time.
Both variables can change how the probe distributes and how long it is retained in a biological system. Dextran size affects the behavior of the carrier, while labeling chemistry determines the detectable signal attached to it. Consequently, probe selection should match the research question because localization, persistence, and signal measurement may vary with conjugate design.
The carrier changes the conjugate’s distribution and retention, so the observed signal reflects both the dye and the dextran-linked design. This distinction matters when a study tracks labeled material through a biological system rather than merely identifying a fluorescent location. The carrier therefore helps maintain a measurable marker for localization and tracing studies.
A suitable workflow begins by selecting dextran size and labeling chemistry according to the intended measurement, then introducing the conjugate into the biological system and examining its uptake or distribution. Researchers can use fluorescence microscopy to detect the signal, including during live-cell imaging, and interpret localization in relation to the experimental design.
These probes can be applied to visualize endocytic trafficking, assess membrane integrity, examine vascular permeability, or trace changes in cell morphology. The same general probe class therefore supports both cellular and tissue-level questions. The relevant readout depends on dextran size, labeling chemistry, and whether the experiment emphasizes localization, retention, or movement.
Fluorescence provides a measurable indication of where labeled material is localized and how that distribution changes during observation. Because uptake commonly occurs through fluid-phase endocytosis and the conjugate can remain in intracellular compartments, signal patterns can inform trafficking and retention. Interpretation should remain tied to probe design and the chosen biological question.