Human Platelet Lysate
Human Platelet Lysate - Related Videos
08:32
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
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Cited by 133 •
2009
Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.
09:13
Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Cited by 5 •
2017
We describe a straightforward method for the isolation of washed platelets from human blood followed by agonist-induced platelet aggregation measurements by turbidimetry. As an example we apply this method for studying the aggregation response of human platelets to collagen after a pre-incubation with the Pannexin1 channel inhibitor Brilliant Blue FCF.
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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Cited by 7 •
2016
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
02:40
Preparation of Platelet-Rich Plasma from Human Peripheral Blood
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2026
Source:Sathish Muthu1,2,31Orthopaedic Research Group, Coimbatore.2Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education.3Department of Orthopaedics, Government Medical College.This video demonstrates the preparation of high-quality Platelet-Rich Plasma (PRP) by screening for platelet count, collecting and centrifuging blood, and isolating platelets. This PRP is used in therapeutic applications for tissue regeneration and healing.
05:49
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Cited by 2 •
2024
Procoagulant platelet formation has been correlated with an increased risk of thrombosis. Presented here is a precise protocol for isolating washed platelets from human blood, intended to quantify the exposure of phosphatidylserine and microvesicle release, which are distinctive features of procoagulant platelets.
FAQs
Breaking open the platelets makes growth-promoting proteins and other bioactive factors available to cultured cells. These components activate signaling pathways associated with cell maintenance and tissue repair, which can support several responses, including proliferation, survival, migration, and differentiation. The combined activity helps explain why the supplement can influence both cell expansion and broader cellular behavior.
Human origin can make the culture environment more biologically relevant when researchers work with primary human cells. It also reduces reliance on animal-derived serum, an important consideration for studies focused on human biology and cell-based therapies. This closer match between supplement source and experimental cells supports research designs intended to model human tissue responses.
Platelet-derived factors can affect more than cell number. Depending on the cultured cell system and controlled laboratory conditions, they may support survival, movement, proliferation, or differentiation. These responses connect the supplement to processes involved in tissue repair and cell maintenance, while also making it relevant to experiments that examine how human cells behave in culture.
A basic workflow begins with human platelets, which are broken open so their growth-promoting proteins and other bioactive factors become available. The resulting supplement is then used under controlled laboratory conditions to support cultured cells. This approach is especially relevant when the goal is to expand mesenchymal stromal cells or other primary human cells.
Researchers may choose Human Platelet Lysate when they need to expand mesenchymal stromal cells or other primary human cells while reducing dependence on animal products. Its human source can also support experiments that seek greater biological relevance to human tissues. These features make it useful in regenerative biology, tissue engineering, disease modeling, and cell-based therapy development.
In biological research, the supplement can support cell proliferation, survival, migration, and differentiation, providing a culture environment suited to studying tissue repair and cell maintenance. Its use extends from routine expansion of primary human cells to broader work in regenerative biology, tissue engineering, disease modeling, and development of cell-based therapies.