Monomer Release
Monomer Release - Related Videos
11:31
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
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Cited by 19 •
2015
Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.
09:47
Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers
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Cited by 32 •
2015
Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.
10:58
Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
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Cited by 7 •
2012
This method describes the combinatorial synthesis of biodegradable polyanhydride film and nanoparticle libraries and the high-throughput detection of protein release from these libraries.
09:02
Using Polystyrene-block-poly(acrylic acid)-coated Metal Nanoparticles as Monomers for Their Homo- and Co-polymerization
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Cited by 1 •
2015
We report protocols for “polymerizing” various types of polymer-encapsulated metal nanoparticles into long chains of “homo-“ and “co-polymers”.
11:02
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
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Cited by 43 •
2015
Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.
FAQs
Hydrolysis uses water to separate the linked units in a polymer by cleaving chemical bonds. This process can occur through chemical treatment or enzyme activity, depending on the biological or material context. The resulting smaller molecules become available for measurement, nutrient recovery, metabolic use, biosynthesis, or further evaluation of polymer breakdown.
Both enzymatic and chemical processes can cleave polymer bonds, but they represent different mechanisms for producing smaller molecular units. Enzymes mediate bond cleavage biologically, whereas chemical treatment achieves it through chemical conditions. Comparing the resulting monomers helps researchers evaluate how a polymer is degraded and whether its breakdown supports biological or material-related objectives.
The released products depend on the polymer being broken down. Proteins can yield amino acids, carbohydrates can yield sugars, and nucleic acids can yield nucleotides. Biodegradable polyesters produce other monomeric building blocks. Identifying these products connects polymer breakdown with nutrient recovery, cellular metabolism, biosynthesis, or assessment of biomaterial degradation.
A study can begin by selecting the polymer and the chemical or enzymatic breakdown process being examined. Researchers then monitor the molecular units liberated from that material and use the measured products to evaluate polymer turnover or degradation. Interpreting which monomers appear provides a link between the breakdown process and its biological or material outcome.
Monomer release is relevant when biological systems recover usable building blocks from larger molecules. Released amino acids, sugars, and nucleotides can enter metabolic pathways or support biosynthesis. In this context, measuring the products of polymer breakdown helps characterize digestive processes and indicates how polymer-derived nutrients may become available for cellular use.
Researchers monitor monomer release to study the breakdown of biodegradable polyesters, controlled drug delivery systems, and other biomaterials. The products provide evidence about polymer turnover and degradation, while their measurement can help assess how a material behaves over time. Such analyses also contribute to understanding the environmental fate of biomaterials after use or disposal.