Muscle Fatty Degeneration
Muscle Fatty Degeneration - Related Videos
06:37
Immunolabelling Myofiber Degeneration in Muscle Biopsies
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Cited by 16 •
2019
Described here is a protocol for direct immunolabelling of necrotic myofibers in muscle cryosections. Necrotic cells are permeable to serum proteins, including immunoglobulin G (IgG). Revealing the uptake of IgG by myofibers allows the identification and quantification of myofibers that undergo necrosis regardless of muscle condition.
06:03
Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs
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2025
This protocol details an in vitro assay for measuring cellular lipid uptake in endothelial cells upon stimulation with BODIPY-C12 and BODIPY-C16 fluorescent analogs of long-chain and very long-chain saturated fatty acids. This method is efficient and adaptable to other cell types, offering a useful approach for studying lipid metabolism.
10:37
Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration
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2023
The present study describes decellularization-based methodologies for visualizing and quantifying intramuscular adipose tissue (IMAT) deposition through intact muscle volume, as well as quantifying metrics of individual adipocytes that comprise IMAT.
05:20
Development of a Rabbit Chronic-Like Rotator Cuff Injury Model for Study of Fibrosis and Muscular Fatty Degeneration
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2023
This study details procedures for establishing a chronic-like rabbit rotator cuff (RC) injury. Specifically, the injury is created in the subscapularis (SSC) muscle-tendon/myotendinous unit to mimic human RC anatomy and pathophysiology, including severe muscle fatty degeneration (FD). This protocol can be applied to study RC injuries and assess regenerative therapies.
08:41
Identification of Fatty Acids in Bacillus cereus
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Cited by 8 •
2016
We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).
FAQs
Muscle size can remain apparently preserved while performance declines because lost contractile fibers may be replaced by connective and adipose tissue. The resulting tissue contains less force-producing machinery, so its dimensions do not necessarily reflect functional capacity. This distinction makes fatty infiltration useful when evaluating muscle quality rather than relying on size alone.
After chronic injury or denervation, damaged fibers may not regenerate fully. Instead, connective and adipose tissue can expand between or within the remaining fibers, producing structural remodeling. Disuse, aging, and disease can create similar conditions in which recovery of contractile tissue is incomplete. The consequence is poorer muscle quality and reduced capacity to regain force.
Fatty infiltration reflects more than a change in appearance: it indicates whether damaged muscle has been restored with contractile tissue or remodeled with noncontractile tissue. As noncontractile tissue increases, force production can fall and recovery may become limited. Researchers therefore use this feature as an indicator of tissue condition in disease and injury studies.
Magnetic resonance imaging and ultrasound provide ways to quantify fatty infiltration, whereas histological analysis examines the remodeled tissue at the microscopic level. These approaches allow biology and clinical researchers to measure structural change rather than infer it only from muscle size or strength. The resulting measurements support assessment of disease, injury, progression, and treatment response.
Researchers can quantify fatty infiltration with magnetic resonance imaging, ultrasound, or histological analysis, then use the measurements to assess a condition or compare muscle status over time. This supports tracking disease progression and judging rehabilitation or therapeutic strategies. The key outcome is an estimate of muscle remodeling that helps distinguish preserved size from preserved muscle quality.
Rotator cuff disease and muscular dystrophy are named applications because both require attention to changes in muscle quality. In these settings, quantifying fatty infiltration helps researchers assess disease status and progression, while also providing an outcome for rehabilitation or therapeutic studies. These measurements connect structural remodeling with the biological question of whether useful contractile tissue is being retained.