Nervous System Pinning
Nervous System Pinning - Related Videos
26:22
Homarus Americanus Stomatogastric Nervous System Dissection
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Cited by 3 •
2009
We describe the fine dissection of the stomatogastric nervous system from the stomach of the American lobster (Homarus americanus).
18:37
Cancer Borealis Stomatogastric Nervous System Dissection
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Cited by 103 •
2009
The stomatogastric nervous system (STNS) of the Jonah crab (C. borealis) can be used for electrophysiology, immunohistochemistry, and cell culture studies. The STNS extraction is done in two parts: the gross and fine dissection.
11:26
In Situ Ca2+ Imaging of the Enteric Nervous System
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Cited by 23 •
2015
The enteric nervous system (ENS) is a network of neurons and glia located in the gut wall that controls intestinal reflexes. This protocol describes methods for recording the activity of enteric neurons and glia in live preparations of ENS using Ca2+ imaging.
01:21
Euler's Formula for Pin-Ended Columns
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2024
In structural engineering, the stability of columns under compressive axial loads is a critical consideration, described as buckling. A typical example involves a column PQ, which is pin-connected at both ends and subjected to a centric axial load F applied at one end, with a reaction force of F' = -F at the other end. Here, it is crucial to understand that when an applied load exceeds the critical load, buckling occurs as the system becomes unstable. To calculate the critical load, envision...
01:21
Nervous System
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2024
The nervous system coordinates body functions through its complex network of nerve cells, enabling sensation and movement. It is divided into two primary parts: the central nervous system (CNS) and the peripheral nervous system (PNS). The CNS is composed of the brain and the spinal cord. The brain acts as the body's control center, processing sensory information and coordinating responses. The spinal cord functions as a major signaling pathway for the brain and the rest of the body. Extending...
FAQs
Fine pins stabilize the surrounding body wall or tissue against a coated dissection dish, limiting movement during examination and manipulation. This support helps researchers expose the central nervous system while reducing excessive motion and potential tissue damage. A stable preparation is especially important when investigators need consistent access for microscopy, staining, or electrophysiological recording.
Physiological saline helps maintain the preparation while the tissue is exposed and secured. Its presence supports continued examination and manipulation of the nervous system during dissection rather than allowing the preparation to become unsuitable for analysis. This condition is therefore important for studies that examine neural structure, neural function, or connections between neurons and muscles.
The coated dish provides a surface against which pins can secure the surrounding body wall or tissue. This arrangement establishes a controlled workspace for exposing the central nervous system and handling the preparation with less unwanted movement. The resulting stability supports detailed visualization and experimental procedures that require the tissue to remain accessible throughout analysis.
A pinned preparation can support microscopy for visualizing neural anatomy, staining for examining tissue features, and electrophysiological recording for studying neural function. It can also help investigators analyze connections between neurons and muscles. These complementary readouts allow researchers to relate visible nervous system structure to functional or anatomical observations within the same general preparation.
The procedure begins by placing the preparation in a coated dissection dish with physiological saline. Fine pins then secure the surrounding body wall or tissue, creating a stable field for exposure of the central nervous system. Once access is established, the preparation can be used for visualization, manipulation, staining, microscopy, or electrophysiological recording.
Biologists use this technique when they need controlled access to nervous tissue in a model organism while preserving preparation stability. It is relevant to neural anatomy studies, electrophysiology, staining, and analyses of neuron-muscle connections. More broadly, the approach contributes to investigations of nervous system development, behavior, and neurological disease by enabling structured examination of neural structure and function.