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In Situ Ca2+ Imaging of the Enteric Nervous System
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Neuroscience
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JoVE Journal Neuroscience
In Situ Ca2+ Imaging of the Enteric Nervous System

In Situ Ca2+ Imaging of the Enteric Nervous System

DOI:
10.3791/52506-v

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11:26 min

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January 29, 2015

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,
1Department of Physiology,Michigan State University

Chapters

  • 00:05Title
  • 02:01Tissue Preparation
  • 05:25Loading Fluo-4 Dye
  • 06:42Imaging and Analysis
  • 09:10Results: Applications of In Situ Ca2+ Imaging of the Enteric Nervous System
  • 10:38Conclusion

Summary

Automatic Translation

Automatic Translation

The enteric nervous system (ENS) is a network of neurons and glia located in the gut wall that controls intestinal reflexes. This protocol describes methods for recording the activity of enteric neurons and glia in live preparations of ENS using Ca2+ imaging.

Tags

Keywords: Enteric Nervous SystemCalcium ImagingMyenteric PlexusNeuronsGliaFluo-4Fluorescence MicroscopyCCD CameraIntestinal FunctionsReflex Behaviors

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