Oil Shear T-junction
Oil Shear T-junction - Related Videos
13:19
Enhanced Oil Recovery using a Combination of Biosurfactants
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Cited by 2 •
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We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.
04:53
A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome
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Cited by 5 •
2010
This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.
10:38
Measurement of the Rheology of Crude Oil in Equilibrium with CO2 at Reservoir Conditions
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Cited by 2 •
2017
A method for measuring the rheology of crude oil in equilibrium with carbon dioxide at reservoir conditions is presented.
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
01:18
Shearing Stress
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2026
Shearing stress, denoted by the Greek letter tau (τ), is stress caused by forces acting transversely on an object. These forces create internal ones within the entity in the plane where the external forces are applied. The resultant of these internal forces is the shear in the section. The average shearing stress can be calculated by dividing the shear by the area of the cross-section. However, this is an average value as the distribution of shearing stress across a section varies. It can...
FAQs
Droplet formation depends on the interaction between the aqueous and oil flow rates, oil-applied shear, and interfacial tension at the phase boundary. The oil stream deforms the incoming aqueous phase until these forces produce pinch-off. Controlling the flows therefore helps generate droplets with more uniform size and creates reproducible compartments for biological materials or reactions.
The immiscible oil provides the surrounding flow that shears the aqueous phase at the junction without mixing with it. This separation allows the aqueous material to break into discrete compartments rather than dispersing into the oil. The resulting droplets can retain cells, proteins, nucleic acids, or reagents in isolated small-volume environments.
Uniform droplets make the experimental compartments more comparable because each can be produced under controlled, reproducible conditions. That consistency supports measurements in which biological contents or reactions must remain isolated from one another. It also helps researchers perform high-throughput analyses, digital assays, and screening while using smaller amounts of sample and reagents.
A biological sample or reaction mixture is introduced as the aqueous phase, while an immiscible oil stream is directed through the intersecting channel. At the junction, controlled flow causes the aqueous phase to pinch off into droplets. The resulting compartments can then support isolated reactions, cell-containing assays, or measurements performed across many small volumes.
The aqueous droplets can encapsulate cells, proteins, nucleic acids, or reagents, depending on the experiment. Compartmentalizing these materials places them into separate, reproducible reaction environments rather than one shared bulk volume. This arrangement is useful when researchers need to examine biological contents individually or combine them with assay reagents under controlled small-volume conditions.
Researchers may choose an Oil Shear T-junction when experiments require isolated reaction environments, precise compartmentalization, or many repeated measurements. Its reported uses include high-throughput single-cell analysis, digital assays, and chemical screening. The approach is especially relevant when reducing sample and reagent consumption while maintaining controlled conditions across numerous droplets is important.