Blunt-end Micropipette
Blunt-end Micropipette - Related Videos
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
08:13
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish
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Cited by 7 •
2021
We modified the Marmarou weight drop model for adult zebrafish to examine a breadth of pathologies following blunt-force traumatic brain injury (TBI) and the mechanisms underlying subsequent neuronal regeneration. This blunt-force TBI model is scalable, induces a mild, moderate, or severe TBI, and recapitulates injury heterogeneity observed in human TBI.
07:02
Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing
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Cited by 1 •
2024
The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.
02:44
Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models
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Cited by 6 •
2024
Here, we describe micropipette-guided drug administration (MDA) as an alternative method to oral gavage that incentivizes the research animal to ingest treatments readily with minimal stress and discomfort.
09:54
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Cited by 8 •
2015
Bacterial mechanosensitive channels can be used as mechanoelectrical transducers in biomolecular devices. Droplet interface bilayers (DIBs), cell-inspired building blocks to such devices, represent new platforms to incorporate and stimulate mechanosensitive channels. Here, we demonstrate a new micropipette-based method of forming DIBs, allowing the study of mechanosensitive channels under mechanical stimulation.
FAQs
Pressure control regulates fluid movement through the glass tool, allowing the operator to aspirate, retain, or release cells and other biological material in a controlled way. This regulation works together with a micromanipulator, which provides precise positioning. Coordinating pressure and movement helps maintain controlled contact with samples during handling and supports reproducible manipulation.
The flattened, nonpointed geometry provides contact without the cutting or puncturing risk associated with a sharp tip. That feature is important when oocytes or embryos must be positioned or held rather than penetrated. By supporting gentler contact, the tool helps preserve sample integrity during manipulation and prepares material for related genetic procedures.
A blunt-end micropipette supports positioning, holding, and transfer, whereas a sharper injection pipette is used alongside it for microinjection. The two tools therefore contribute different mechanical functions within the same workflow. Separating gentle sample handling from penetration allows researchers to manipulate cells or embryos before or during genetic intervention with greater control.
The essential setup combines the glass micropipette with a micromanipulator and a pressure system. The micromanipulator controls the tool’s position, while the pressure system regulates fluid movement through it. Together, these components let an operator bring the blunt tip into controlled contact with a sample and aspirate, hold, or transfer material.
Researchers may use it when oocytes, embryos, or other cells must be positioned, held, or transferred during genetic workflows. It can also support microinjection or biopsy procedures by stabilizing and managing biological material while a sharper pipette performs a different task. Its value is greatest when controlled handling and sample integrity are important.
Careful use can improve the consistency of cell and embryo handling, reduce avoidable damage from puncturing or cutting, and support reproducible genetic manipulation and analysis. These benefits arise from combining gentle blunt-tip contact with controlled positioning and pressure. The tool therefore contributes to workflow reliability, even when it is used alongside other microtools.