Yap Taz
Yap Taz - Related Videos
13:05
De Novo Generation of Somatic Stem Cells by YAP/TAZ
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Cited by 4 •
2018
Availability of somatic SCs is crucial for regenerative medicine, disease modeling and to gain insight into SC properties. Here we present experimental strategies to reprogram, in vitro, differentiated adult cells into their corresponding expandable tissue-specific stem/progenitor cells by the transient expression of the single transcriptional co-activator YAP.
10:57
Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children
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Cited by 59 •
2012
Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.
07:49
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
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Cited by 4 •
2019
In this article, we presented a set of practical and feasible methods for characterizing disease-related mutants of RAF family kinases, which include in vitro kinase assay, RAF co-activation assay, and complementary split luciferase assay.
08:54
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
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Cited by 2 •
2021
We designed and constructed a mobile laboratory to measure respiration rates in isolated mitochondria of wild animals captured at field locations. Here, we describe the design and outfitting of a mobile mitochondrial laboratory and the associated laboratory protocols.
11:26
Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
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Cited by 4 •
2014
An integrated device, incorporating a dye-sensitized solar cell and triplet-triplet annihilation up-conversion unit was produced, affording enhanced light harvesting, from a wider section of the solar spectrum. Under modest irradiation levels a significantly enhanced response to low energy photons was demonstrated, yielding a record figure of merit for dye-sensitized solar cells.
FAQs
Phosphorylation acts as a control point for YAP/TAZ activity. When upstream Hippo pathway kinases phosphorylate these coactivators, they are retained in the cytoplasm or directed toward degradation, limiting access to nuclear gene-regulatory machinery. This switch allows signaling changes to alter transcription and, consequently, cellular behaviors such as proliferation, survival, and differentiation.
Tissue architecture and mechanical cues provide information that can alter Hippo pathway activity. Reduced pathway activity favors YAP/TAZ entry into the nucleus, where they can affect transcription, whereas active signaling restricts them outside the nucleus or promotes their removal. This connection helps cells adjust growth, differentiation, and survival to their tissue environment.
The interaction with TEAD transcription factors gives nuclear YAP/TAZ a route to activate specific target genes. Without this transcriptional partnership, changes in YAP/TAZ localization would not directly produce the same gene-expression response. Studying this interaction therefore helps connect altered signaling or mechanics with downstream effects on organ growth, cell survival, and tissue behavior.
Mutations can disrupt components that control YAP/TAZ signaling or change how cells respond to pathway inputs. Such genetic alterations may shift the balance between cytoplasmic retention, degradation, and nuclear activity, changing target-gene expression. In genetic research, this provides a framework for linking mutations with abnormal development, regeneration, fibrosis, or cancer-related tissue changes.
A useful study can connect genetic changes with signaling state, YAP/TAZ localization, and resulting gene-expression effects. Researchers may ask whether pathway activity promotes cytoplasmic retention or degradation, or instead permits nuclear entry and TEAD-associated transcription. Relating these molecular changes to proliferation, survival, differentiation, or organ growth clarifies the biological consequence of a variant or altered signal.
YAP/TAZ analysis is relevant to development, regeneration, fibrosis, and cancer research because each area can involve altered growth, differentiation, survival, or tissue organization. The pathway provides a way to study how genetic mutations, signaling changes, and mechanical conditions converge on gene expression. These comparisons can reveal shared regulatory principles across different biological and disease contexts.
Mapping the steps that control YAP/TAZ, including upstream kinase activity, cytoplasmic retention, degradation, nuclear entry, and TEAD partnership, identifies points where abnormal regulation might be addressed. Research can then relate those control points to disease-associated gene expression and tissue behavior. This mechanistic understanding supports investigation of potential therapeutic targets in conditions such as fibrosis and cancer.