B Cell Electroporation
B Cell Electroporation - Related Videos
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
08:39
Electroporation of Functional Bacterial Effectors into Mammalian Cells
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Cited by 7 •
2015
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
13:31
Single Cell Electroporation in vivo within the Intact Developing Brain
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Cited by 26 •
2008
Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.
05:40
In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Cited by 4 •
2016
Here, we present an in vivo technique for gene transfer to Schwann cells (SCs) in the rodent sciatic nerve. This simple technique is useful for investigating signaling mechanisms involved in the development and maintenance of myelinating SCs.
25:06
In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
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Cited by 30 •
2009
Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.
FAQs
Electrical pulses create a short-lived change in the B-cell plasma membrane, producing transient pores rather than a permanent opening. This temporary state allows material outside the cell to cross the membrane, after which the membrane reseals as the cell recovers. The timing matters because delivery depends on this brief window while preserving the manipulated cell for later immunological analysis.
B cell electroporation can deliver nucleic acids, proteins, and other molecules into B lymphocytes. This range lets investigators select cargo according to the experimental question, such as examining gene expression with nucleic acids, manipulating cellular activity with proteins, or testing another molecular input. The method therefore supports more than a single type of intracellular intervention.
Transient disruption provides access to the cell interior without requiring the membrane to remain open. Because the pores are temporary and the membrane reseals during recovery, researchers can study B cells after introducing experimental material rather than analyzing only the immediate entry event. This feature is especially useful when later immune responses, signaling, or gene-expression changes are the intended outcomes.
A basic workflow brings B lymphocytes into contact with the chosen extracellular cargo, applies brief electrical pulses, and then allows the cells to recover as their membranes reseal. Researchers can subsequently assess the effect of the introduced material. The source material does not specify pulse settings or equipment, so those experimental parameters should be selected for the particular B-cell study.
Researchers may choose B cell electroporation when they need controlled manipulation of B lymphocytes for gene expression studies, antigen-response analysis, or signaling investigations. Its value is particularly apparent when the cells are difficult to transfect by other approaches. The resulting access to intracellular experimental material helps connect a defined molecular intervention with a measurable immune-cell response.
In immunology and infection research, the technique supports analysis of how B cells respond to antigen or pathogen exposure. Investigators can introduce material, examine changes in gene expression or signaling, and study consequences for immune function. It also contributes to antibody engineering and experimental strategies related to vaccines and therapeutic antibodies, extending the method from basic mechanisms to translational research.