Cd40-activated B Cells
Cd40-activated B Cells - Related Videos
13:27
Generation of Human CD40-activated B cells
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Cited by 36 •
2009
In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.
12:24
Murine Model of CD40-activation of B cells
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Cited by 13 •
2010
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
18:48
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
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Cited by 1 •
2017
Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.
05:30
Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence
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2024
This protocol describes a unique method for detecting the binding between compounds and protein molecules, offering the advantages of minimal protein sample loss and high data accuracy.
13:38
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Cited by 21 •
2012
FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.
FAQs
Engagement of CD40 initiates intracellular signals that support B-cell proliferation and survival while directing later functional changes. These signals help determine whether cells undergo immunoglobulin class switching or differentiate toward antibody-secreting or memory-cell states. The pathway therefore connects recognition of helper T-cell support with durable and diversified B-cell responses relevant to adaptive immunity.
CD40 signaling establishes an activation framework, but cytokines further shape the resulting B-cell response. Their influence can affect the balance among proliferation, survival, immunoglobulin class switching, and differentiation into antibody-secreting or memory cells. Including cytokine conditions in an experimental model allows investigators to examine how different immune signals modify the outcome of T-cell-dependent B-cell activation.
These cells provide a controlled way to examine the consequences of the CD40 pathway without treating the response as a single undifferentiated activation event. Investigators can focus on how helper T-cell-associated signaling supports B-cell expansion, antigen presentation, antibody-related differentiation, and memory formation. That controlled context helps connect cellular mechanisms with broader adaptive immune responses.
A model can be evaluated for B-cell proliferation, survival, immunoglobulin class switching, and differentiation into antibody-secreting or memory cells. It can also support analysis of antigen presentation and the effects of additional cytokine signals. Together, these readouts show whether stimulation promotes expansion, functional antibody responses, longer-lasting cellular states, or altered immune communication.
A typical conceptual workflow begins by stimulating B lymphocytes through CD40, then examining how the cells respond under defined cytokine conditions. Researchers can track expansion and survival before assessing class switching and differentiation into antibody-secreting or memory cells. Adding antigen-presentation measurements extends the workflow from intrinsic B-cell activation to interactions relevant to adaptive immune defense.
They are used when investigators need to investigate T-cell-dependent activation, antigen presentation, or host defense in a controlled cellular system. The model also supports research on vaccine responses and immune deficiencies, where altered antibody-related activity is important. Because the pathway can be examined as both stimulatory and regulatory, it is relevant to strategies that enhance or restrain antibody-mediated immunity.