Luxab Reporter Genes
Luxab Reporter Genes - Related Videos
02:11
Reporter Genes
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2021
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. Commonly used reporter...
04:25
Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene
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2025
This video demonstrates an in vivo assay in bacteria to study the interaction of RNA-binding proteins (RBPs) with RNA. The bacterial cells are transformed with two plasmid constructs — a binding-site plasmid encoding an mRNA containing a fluorescent reporter gene downstream of an RBP-binding site — while an RBP plasmid expresses the RBP proteins under the control of an inducer. Upon inducer-mediated increase in RBP production, the RBPs bind to the mRNA, resulting in translational repression of...
12:58
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
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Cited by 13 •
2015
Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
13:48
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
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Cited by 6 •
2008
Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-GloTM Luciferase Assay System from Promega.
10:48
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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Cited by 5 •
2014
We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.
FAQs
The linked promoter controls expression of the luxAB coding sequences, which produce the alpha- and beta-subunits of bacterial luciferase. Together, these subunits support oxidation of reduced flavin mononucleotide and a long-chain aldehyde when oxygen is available. The resulting light provides a measurable readout of activity in living cells, allowing promoter behavior to be followed without destroying the sample.
These components are reactants required for the luciferase reaction rather than interchangeable features of the reporter. Oxygen, reduced flavin mononucleotide, and a long-chain aldehyde participate in the oxidation process that releases light. Their involvement explains why the measured signal represents an active biochemical reaction and provides the chemical basis for detecting expression from the linked promoter.
Signal intensity provides a measurable indication of luciferase expression and therefore activity of the promoter connected to luxAB. Changes in brightness can be examined as promoter activity changes in living cells, making the reporter useful for tracking regulation over time. In infection studies, this connects molecular gene activity with changing pathogen responses during host interaction or treatment.
A study can detect the bioluminescent signal in living cells at multiple observation points rather than relying only on a single endpoint. Because detection is noninvasive, the same infection or cell population can be followed as its signal changes. This time-resolved approach helps reveal infection progression, responses to host defenses, and treatment-associated changes.
The signal can be used to monitor pathogen viability, colonization, and dissemination, providing several complementary views of infection rather than a single molecular measurement. Tracking these features over time helps researchers examine where infection develops and how it progresses. The approach is particularly useful when spatial or temporal changes in pathogen-associated signal matter.
Researchers can follow changes in reporter signal while pathogens encounter host defenses or antimicrobial treatments. These changes can be examined alongside the study condition to assess effects on pathogen-associated activity, viability, or infection progression. This makes the reporters useful for connecting immune pressure or treatment exposure with dynamic host-microbe outcomes.