Immunology and Infection
A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.
Summary May 15th, 2014
We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.