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JoVE Journal
Immunology and Infection

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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Article DOI: 10.3791/51522
May 15th, 2014 •
1, 1, 1, 1
1Department of Microbiology, Boston University School of Medicine

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May 15th, 2014

We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.

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