Breast Cancer Metastasis
Breast Cancer Metastasis - Related Videos
08:11
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
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Cited by 83 •
2016
An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.
07:47
Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis
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Cited by 7 •
2016
Here we describe the model and approach to study functions of pulmonary alveolar macrophages in cancer metastasis. To demonstrate the role of these cells in metastasis, the syngeneic (4T1) model of breast cancer in conjunction with the depletion of alveolar macrophage with clodronate liposomes was used.
07:13
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
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Cited by 27 •
2014
Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.
09:53
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
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Cited by 18 •
2016
We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.
12:23
Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
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Cited by 16 •
2012
In the pathogenesis of bone metastasis, angiogenesis is a crucial process and therefore represents a target for imaging and therapy. Here, we present a rat model of site-specific breast cancer bone metastasis and describe strategies to non-invasively image angiogenesis in vivo using magnetic resonance imaging, volumetric computed tomography and ultrasound.
FAQs
The process unfolds as a linked cascade rather than a single event. Malignant cells first invade surrounding tissue, then enter blood or lymphatic vessels, withstand transport, leave the circulation in another tissue, and adapt to a supportive local environment. Examining these stages separately helps researchers determine where dissemination may be interrupted or where secondary growth becomes established.
Blood and lymphatic vessels provide routes through which malignant breast cells can leave the primary tumor region and travel elsewhere in the body. Entry into either system is followed by survival during transport and exit into a new tissue. Studying both routes is important because dissemination can involve more than one vascular pathway.
A distant tissue must provide conditions that allow disseminated cells to adapt and establish a secondary tumor. This supportive local setting is part of the metastatic process, not merely a passive location where cells arrive. Investigating organ-specific colonization therefore focuses attention on why some tissues may support metastatic growth more effectively than others.
Biomarkers can help researchers identify features associated with a greater likelihood of metastatic spread. Their value comes from connecting measurable biological characteristics with stages of the dissemination cascade or with organ-specific colonization. In medicine, this information may support efforts to distinguish metastatic risk and guide research into strategies that limit future disease progression.
Examining the full cascade can show how cells move from local invasion to distant colonization and how they adapt after reaching another tissue. This broader view links cellular behavior with the development of secondary tumors and organ-specific patterns. It also provides a framework for investigating biomarkers and for identifying stages that may be vulnerable to treatment.
Research supports two complementary treatment goals: preventing malignant cells from completing the dissemination cascade and controlling disease after secondary tumors have become established. The first goal focuses on blocking steps in spread, while the second addresses existing metastatic disease. Keeping these goals distinct helps align therapeutic research with the stage of disease being targeted.