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Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis
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JoVE Journal Medicine
Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis

Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis

DOI:
10.3791/54306-v

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07:47 min

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June 26, 2016

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, , , , ,
1Department of Immunotherapeutics and Biotechnology, School of Pharmacy,Texas Tech University Health Science Center, 2Merck Research Labs, 3Department of Surgery,Memorial Sloan Kettering Cancer Center

Chapters

  • 00:05Title
  • 00:54Murine 4T1/4T1-GFP Cell Injection, Tumor Growth Monitoring and 4T1-GFP Cell Imaging
  • 02:05Intranasal Liposome Administration and Lung Metastasis Evaluation
  • 03:20Alveolar Macrophage Flow Cytometric Analysis
  • 06:08Results: Representative Tumor Growth and Metastatic Lung Burden Assessment and Analysis
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe the model and approach to study functions of pulmonary alveolar macrophages in cancer metastasis. To demonstrate the role of these cells in metastasis, the syngeneic (4T1) model of breast cancer in conjunction with the depletion of alveolar macrophage with clodronate liposomes was used.

Tags

Keywords: Alveolar MacrophagesBreast CancerMetastasisSyngeneic ModelTumor ImmunologyCancer MetastasisPulmonary Alveolar MacrophagesTumor CellsTumor InoculationTumor VolumeFluorescence MicroscopyLiposome DeliveryLung Surface Metastases

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