Testicular Tissue
Testicular Tissue - Related Videos
15:33
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
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Cited by 20 •
2013
A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.
07:34
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
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Cited by 14 •
2014
The material here describes a method developed to preserve the three-dimensional chromatin structure of testicular germ cells. This has been termed the three-dimensional (3D) slide method. This method improves sensitivity for detection of subnuclear structures and is applicable for immunofluorescence, DNA, and RNA fluorescence in situ hybridization (FISH).
10:41
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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Cited by 16 •
2012
Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.
11:53
Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
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Cited by 25 •
2019
Here, we present a protocol for the reproducible generation of porcine testicular organoids with testis specific tissue architecture using the commercially available microwell culture system.
07:48
Working with Human Tissues for Translational Cancer Research
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Cited by 4 •
2015
Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.
FAQs
Spermatogenesis depends on organized activity within the seminiferous tubules, where germ cells progress toward sperm formation. Sertoli cells provide the surrounding structural and metabolic support required by these developing cells. Examining this relationship helps investigators determine whether impaired sperm production reflects disruption of germ-cell development, inadequate support, or broader tissue dysfunction.
Luteinizing hormone stimulates interstitial Leydig cells to produce testosterone, linking hormonal signaling with testicular activity. Testosterone is essential to the reproductive functions supported by the testes, so altered Leydig-cell activity can indicate a hormonal disorder. Analysis of this compartment therefore complements evaluation of seminiferous-tubule changes when investigating reproductive or endocrine problems.
The seminiferous and interstitial compartments provide different information. Changes involving germ cells or Sertoli-cell support may point toward impaired sperm development, whereas abnormalities affecting Leydig cells may be associated with disturbed testosterone production. Considering both regions prevents tissue assessment from focusing on fertility or hormone function in isolation.
In infertility assessment, tissue analysis can examine the condition of germ cells, seminiferous tubules, Sertoli-cell support, and Leydig-cell activity. These observations help relate microscopic tissue status to sperm-production problems or hormonal abnormalities. The resulting information can distinguish reproductive dysfunction involving developmental processes from changes associated with testosterone secretion.
Evaluation may be relevant to suspected tumors, trauma, hormonal disorders, or developmental abnormalities. Each situation can affect testicular structure or function in a different way, making tissue assessment useful for identifying the involved cellular regions. In medicine, these findings contribute to understanding disease-related damage and its potential effects on reproductive capacity.
Laboratory models help investigate germ-cell biology, fertility preservation, reproductive toxicology, and the effects of disease or treatment on testicular function. They provide a controlled setting for examining how tissue components respond to specific conditions. This research context can clarify mechanisms of reproductive injury and support development of strategies to preserve function.