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Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
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Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Article DOI: 10.3791/60387-v • 11:53 min • October 3rd, 2019
October 3rd, 2019 •
1, 3,4, 2, 2,3,4, 1,2
1Biochemistry and Molecular Biology, University of Calgary, 2Comparative Biology and Experimental Medicine, University of Calgary, 3Biomedical Engineering Graduate Program, University of Calgary, 4Alberta Diabetes Institute, University of Alberta

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Summary
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Here, we present a protocol for the reproducible generation of porcine testicular organoids with testis specific tissue architecture using the commercially available microwell culture system.

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Testicular Organoids Microwell Culture Testicle Morphogenesis Drug Screening Toxicity Screening Reproducible Technique Low Cell Requirement Testis-specific Architecture Pluripotent Stem Cells Pancreatic Islet Cells Enzymatic Digestion Procedure Anna Voigt Sterile Beaker PBS Penicillin Streptomycin Autoclaved Scissors Forceps Tunica Vaginalis Epididymis Longitudinal Axis DMEM
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