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Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
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Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

DOI:
10.3791/60387-v

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11:53 min

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October 03, 2019

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, , , ,
1Biochemistry and Molecular Biology,University of Calgary, 2Comparative Biology and Experimental Medicine,University of Calgary, 3Biomedical Engineering Graduate Program,University of Calgary, 4Alberta Diabetes Institute,University of Alberta

Chapters

  • 00:04Title
  • 00:51Testis Tissue Enzymatic Digestion
  • 05:22Germ Cell Enrichment
  • 06:52Preparation of Cells and Microwells
  • 08:21Generation of Testicular Organoids
  • 09:51Results: Testis-specific Tissue Architecture
  • 10:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the reproducible generation of porcine testicular organoids with testis specific tissue architecture using the commercially available microwell culture system.

Tags

Keywords: PorcineTestisTesticular OrganoidsTestis-specific ArchitectureMicrowell CultureTestis MorphogenesisDrug ScreeningToxicity ScreeningEnzymatic DigestionCollagenaseDMEMCell Isolation

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