Mouse Drg Sections
Mouse Drg Sections - Related Videos
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
11:13
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Cited by 19 •
2015
The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.
04:48
Preparation of Mouse Vomeronasal Organ for Vibratome Sectioning
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2025
This video outlines the isolation of a mouse vomeronasal organ (VNO) and tissue preparation for vibratome sectioning. Sections are used to characterize the biophysical and physiological attributes of a specific group of sensory neurons.
FAQs
The arrangement of cells within a section helps researchers interpret sensory neurons in relation to their surrounding tissue rather than as isolated structures. Maintaining this organization supports examination of neuronal morphology, neuronal markers, and satellite glial cells in their tissue context. It also makes tissue-level changes associated with injury or disease easier to evaluate.
Researchers can assess the morphology of peripheral sensory neurons, the presence of neuronal markers, and the organization of satellite glial cells. Examining these features together provides a broader view of ganglion tissue, allowing cellular changes to be considered across both neurons and neighboring support cells rather than focusing on a single cell population.
Changes visible in the sections can be related to sensory processes studied in neuroscience, including pain and mechanosensation. The tissue provides an intermediate level of analysis between individual cellular markers and whole-system function. This makes it possible to examine how alterations in sensory neurons or surrounding cells may accompany injury, disease, or changes in peripheral sensory signaling.
Preparation begins with fixation of the dorsal root ganglion tissue, followed by embedding and precise sectioning. These stages stabilize the sample and produce thin slices suitable for downstream analysis. Maintaining accuracy during sectioning is important because the resulting tissue must retain enough structure for stains, immunolabeling, or microscopy to reveal cellular organization.
Stains, immunolabeling, and microscopy provide complementary ways to analyze prepared ganglion tissue. They can support visualization of cellular organization, neuronal morphology, neuronal markers, and satellite glial cells. Selecting among these approaches depends on the feature under study, while combining tissue preparation with imaging enables researchers to relate visible structures to injury or disease responses.
These sections are useful when a study requires tissue-level examination of the peripheral sensory system. Applications include investigating pain, mechanosensation, neuroinflammation, and peripheral nerve regeneration, as well as examining responses to injury or disease. Their value lies in connecting changes in sensory neurons and surrounding cells with broader questions about somatosensory function.