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Immunology and Infection

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JoVE Journal Immunology and Infection
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
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Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Article DOI: 10.3791/2659-v • 08:51 min • June 2nd, 2011
June 2nd, 2011 •
1, 1, 1, 2, 1
1Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation, Helmholtz Center for Infection Research

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We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

Tags

Phagocytosis NET-formation Neutrophils Infected Lungs 2-photon Microscopy Gas Exchange Defense Strategies Humoral Immune Mechanisms Cellular Immune Mechanisms Recruitment Of Neutrophils Cytotoxic Chemicals Extracellular DNA-NETs Bacteria Fungi Lung Infection Viable Lung Slices Intratracheal Infection Aspergillus Fumigatus Multicolor Time-lapse Microscopy
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