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Genomik DNA 5-hydroxymethylcytosine seçici Yakalama

Article DOI: 10.3791/4441-v • 06:26 min • October 5th, 2012
October 5th, 2012 •
1, 2, 2, 1
1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

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Açıklanan kolay ve yoğunluk bağımsız zenginleştirme için bir biyotin bağlayıcının aktarmak tıkırtı kimya ve ardından, 5-HMC için bir azit-glikoz aktarmak için β-glukoziltransferaz (β-GT) ile iki aşamalı bir etiketleme bir işlemdir. Bu etkili ve spesifik bir etiketleme yöntemi nesil dizileme ile son derece düşük arka plan ve yüksek verimlilik epigenomic haritalama ile 5-HMC zenginleşmesine olanak sağlar.

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Genetik Sayı 68 Kimya Biyofizik 5-Hydroxymethylcytosine kimyasal etiketleme genomik DNA yüksek hacimli dizileme
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