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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Article DOI: 10.3791/51857-v • 10:24 min • October 28th, 2014
October 28th, 2014 •
1, 2
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

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Summary
Automatic Translation

Calcium is involved in numerous physiological and pathophysiological signaling pathways. Live cell imaging requires specialized equipment and can be time consuming. A quick, simple method using a flow cytometer to determine relative changes in cytosolic calcium in adherent epithelial cells brought into suspension was optimized.

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Cytosolic Calcium Measurements Renal Epithelial Cells Flow Cytometry Cellular Processes Calcium Signaling Calcium Binding Dyes Live Cell Imaging Fluorescence Intensity Emission Wavelength Alternative Detection Methods Immunological Cells Non-immunological Adherent Cells Fluorescence Microplate Reader Epithelial Cells Renal Proximal Tubule Cells Cell Permeable Calcium Binding Dye
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