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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
JoVE Journal
Biologie
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JoVE Journal Biologie
TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

DOI:
10.3791/52985-v

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10:26 min

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September 10, 2015

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, , , ,
1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center

Kapitel

  • 00:05Titel
  • 02:09Amplification of TRAP Line
  • 04:29Lysate Preparation
  • 06:10Pre-absorption
  • 07:03Immunopurification
  • 08:35Results: Quality and Specificity of TRAP-Isolated RNA from Slouch-GAL4>UASRpL10A-EGFP Embryos
  • 09:56Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

Tags

TRAPTranslating Ribosome Affinity PurificationRare Cell PopulationsDrosophila EmbryosMRNA TranslationCell-type-specificTranslatome AnalysisPolysomesTagged Ribosomal SubunitDevelopmental ProcessesDisease ProgressionCell-specific Gene ExpressionMicroarrayRNA-seqGal4/UAS SystemMuscle Cells

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