JoVE Journal
Biology
Biology
Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.
Kapitel
Summary
Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.