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Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
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Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Article DOI: 10.3791/54258-v • 08:23 min • July 10th, 2016
July 10th, 2016 •
1,3, 1,3, 1,3, 2,3,4, 1,3,4
1Department of Biological Engineering, Inha University, 2Department of Mechanical Engineering, Inha University, 3Biohybrid Systems Research Center (BSRC), Inha University, 4Convergent Research Center for Metabolism and Immunoregulation (CRCMI), Inha University

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Summary
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We demonstrate a storable, transportable lipid bilayer formation system. A lipid bilayer membrane can be formed within 1 hr with over 80% success rate when a frozen membrane precursor is brought to ambient temperature. This system will reduce laborious processes and expertise associated with ion channels.

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Automated Lipid Bilayer Membrane Formation Polydimethylsiloxane Thin Film Nanobiosensor Field Membrane-protein Interactions Buffer Solution Potassium Chloride Tris Hydrochloride EDTA Lipid Solution Pre-painting Membrane Formation Undecane Hexadecane PDMS Thin Film Prepolymer Curing Agent Spin Coat Vacuum Desiccator
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