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Neuroscience

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JoVE Journal Neuroscience
使用荧光素二乙酸酯 - 碘化丙啶双重染色在小脑颗粒神经元培养中评估神经元活力
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使用荧光素二乙酸酯 - 碘化丙啶双重染色在小脑颗粒神经元培养中评估神经元活力

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

Kapitel

Summary
Automatic Translation

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Click here for the English version.

该协议描述了如何使用荧光素二乙酸酯（FDA）和碘化丙啶（PI）双重染色在培养的小脑颗粒神经元中准确测量神经元活力，这是一种初级神经元培养物，用作神经科学和神经药理学研究中的体外模型。

Tags

神经科学，第123期，小脑颗粒神经元，荧光素二乙酸酯，碘化丙啶，细胞活力，胶质细胞，荧光显微镜
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