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Journal / Neuroscience / 小鼠小脑颗粒神经元神经元死亡和变性的模型研究…
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Neuroscience

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小鼠小脑颗粒神经元神经元死亡和变性的模型研究
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小鼠小脑颗粒神经元神经元死亡和变性的模型研究

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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本协议描述了一种简单的方法, 分离和培养的小鼠脑颗粒神经元 (CGNs) 从6-7 天的老幼崽, 有效的转导 CGNs 的损失和增益的功能研究, 并建模 NMDA 诱导神经元兴奋,低诱导的细胞死亡, DNA 损伤, 和氧化应激使用相同的培养模式。

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神经科学 问题 129 小脑 小脑颗粒神经元 (CGN) 腺病毒 慢转导 兴奋 n-甲基-d-天门冬氨酸 (NMDA) 凋亡 氯化钾 (氯化钾) 喜树碱 过氧化氢 (H2O2)
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