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Journal / Biochemistry / Provberedning för Mass Spectrometry-baserade…
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Biochemistry

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Provberedning för Mass Spectrometry-baserade identifiering av RNA-bindande regioner

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Vi beskriver ett protokoll för att identifiera RNA-bindande proteiner och kartlägga deras RNA-bindande regioner i levande celler med hjälp av UV-medierad photocrosslinking och masspektrometri.

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Biokemi problemet 127 Protein-RNA interaktioner RNA-bindande domän RNA-bindande region masspektrometri icke-kodande RNAs UV crosslinking
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