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Journal / Biochemistry / 質量分析を用いた識別 RNA…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
質量分析を用いた識別 RNA 結合領域のための試料作製
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質量分析を用いた識別 RNA 結合領域のための試料作製

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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RNA 結合蛋白質を識別し、紫外線による光架橋と質量分析法を用いた生細胞におけるその RNA 結合領域をマップするためのプロトコルについて述べる。

Tags

生化学、問題 127、RNA 蛋白質の相互作用、RNA 結合ドメイン、RNA 結合領域、質量分析法、Rna、UV 架橋
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